Journal: Molecular Medicine Reports

Article Title: Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air-blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF-κB/IκBα pathway

doi: 10.3892/mmr.2026.13797

Figure Lengend Snippet: Injury of mucus hypersecretion and oxidative damage in all groups. (A) AB-PAS staining images of the airway in all groups (AB-PAS; ×200 magnification and ×400 magnification). Arrows indicate goblet cells. (B) The expression level of MUC5AC and MUC5B in the airway tested using immunohistochemistry (×200 magnification). (C) The expression of IL-6, IL-10, TNF-α, T-SOD, MDA, MMP-9 and TIMP-1 in lung tissue and serum of rats in all groups. The values are expressed as mean ± SE; (n=8). *P<0.05 and **P<0.01 vs. normal group. AB-PAS, Alcian blue-periodic acid-schiff; MUC5AC, mucoprotein-5AC; MUC5B, mucoprotein-5B; T-SOD, total superoxide dismutase; MDA, malondialdehyde; MMP-9, matrix metalloproteinase 9; TIMP-1, tissue inhibitor of metalloproteinases 2; IOD, integrated optical density.

Article Snippet: The secretion of IL-6 (Rat IL-6 ELISA Kit; cat. no. E-EL-R0015; Elabscience Bionovation Inc.), IL-10 (Rat IL-10 ELISA Kit; cat. no. E-EL-R0016; Elabscience Bionovation Inc.), malondialdehyde (MDA) (MDA assay kit; cat. no. A003-1-2; Nanjing Jiancheng Bioengineering Institute), total superoxide dismutase (T-SOD) (SOD assay kit; cat. no. A001-3-2; Nanjing Jiancheng Bioengineering Institute), MMP-9 (Rat MMP-9 ELISA Kit; cat. no. E-EL-R3021; Elabscience Bionovation Inc.) and tissue Inhibitors of metalloproteinase 1 (TIMP-1 ELISA Kit; cat. no. E-EL-R0540; Elabscience Bionovation Inc.) in the lung tissue homogenate was measured using ELISA kits, following the manufacturer's instructions.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: Biochemistry and Biophysics Reports

Article Title: Evaluation of a research use only (RUO) TIMP-1 assay for use with the automated Alinity i platform

doi: 10.1016/j.bbrep.2026.102509

Figure Lengend Snippet: Linearity performance of the Alinity i TIMP-1 immunoassay across the analytical measuring interval Observed concentrations for gravimetrically prepared dilution levels are plotted against expected values. All levels demonstrated a deviation from linearity within ±10%, confirming linear response throughout the validated range.

Article Snippet: Here, we describe reformulation of the RUO TIMP-1 immunoassay for migration to the high-throughput automated Alinity i platform (Abbott Laboratories) and assessment of its analytical performance.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Evaluation of a research use only (RUO) TIMP-1 assay for use with the automated Alinity i platform

doi: 10.1016/j.bbrep.2026.102509

Figure Lengend Snippet: Distribution of TIMP-1 concentrations in normal plasma samples (n = 100) and cardiac specimens with elevated troponin-I (n = 88) The normal donor cohort establishes the physiological concentration range, while cardiac specimens exhibit higher TIMP-1 levels.

Article Snippet: Here, we describe reformulation of the RUO TIMP-1 immunoassay for migration to the high-throughput automated Alinity i platform (Abbott Laboratories) and assessment of its analytical performance.

Techniques: Clinical Proteomics, Concentration Assay